کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530519 1549312 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ReviewQuantifying lipid changes in various membrane compartments using lipid binding protein domains
ترجمه فارسی عنوان
بررسی تغییرات چربی در محفظه های غشایی مختلف با استفاده از دامنه های پروتئینی لیدینگ اتصال دهنده
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


- Exact lipid distribution between various membranes is largely unknown.
- Many proteins contain lipid binding modules with some specificity.
- Some of these modules can be used to detect lipid distribution in live cells.
- Quantification of lipid changes using these tools is increasingly desirable.

One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid metabolism and exceptional regulatory importance in the control of almost all aspects of cellular functions created the need for tools to visualize their localizations and dynamics at the single cell level. However, there is also an increasing need for methods to determine the cellular distribution of other lipids regulatory or structural, such as diacylglycerol, phosphatidic acid, or other phospholipids and cholesterol. This review will summarize recent advances in this research field focusing on the means by which changes can be described in more quantitative terms.

114

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 64, June 2017, Pages 72-82
نویسندگان
, , , , , ,