کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5532273 1549666 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperERK activated by Histamine H1 receptor is anti-proliferative through spatial restriction in the cytosol
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Research paperERK activated by Histamine H1 receptor is anti-proliferative through spatial restriction in the cytosol
چکیده انگلیسی


- Histamine H1 receptor activation causes ERK phosphorylation.
- Phospho-ERK generated through histamine stimulation is restricted in the cytoplasm.
- Cytosolic pErk does not generate pro-proliferative responses and shows different spatio − temporal dynamics.
- ROS production post histamine stimulation is through a H1R → → → pERK cascade.

Histamine, a primary mediator of allergic responses, elicits its effects by activating specific receptors belonging to the GPCR family in target cells. Activation of histamine receptor can activate MAP kinases as recorded by monitoring the phosphorylation of extracellular signal regulated kinase (ERK). Despite this, ERK phosphorylation does not translate into pro-proliferative changes after histamine stimulation in HeLa cells. Here we show that histamine H1 receptor activation mediates MAPK activation through PLCβ, Src, PKCδ and MEK pathway, but does not lead to nuclear relocalization of phospho-ERK (pERK), classically associated with pro-proliferative changes. Live cell imaging, FRET and FRAP measurements along with functional analysis reveal that pERK generated by histamine activation is physically and functionally restricted in the cytosol and the findings report a spatial regulation of MAPK cascade activated non-canonically through GPCRs unlike its canonical activation by EGF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 95, Issue 12, December 2016, Pages 623-634
نویسندگان
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