کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5534092 | 1550837 | 2017 | 17 صفحه PDF | دانلود رایگان |

- Ghrelin and GHS-R1a colocalize digestive enzymes in goldfish intestine and hepatopancreas.
- Ghrelin induces sucrase-isomaltase, aminopeptidase A and lipoprotein lipase expression in vitro.
- GHS-R1a is critical for ghrelin-induced upregulation of digestive enzymes.
- Ghrelin actions on digestive enzymes might be partly mediated by PLC/PKC and AC/PKA signaling pathways.
Ghrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. This study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (Carassius auratus). Immunofluorescence microscopy found colocalization of ghrelin, GHS-R1a and the digestive enzymes sucrase-isomaltase, aminopeptidase A, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. In vitro ghrelin treatment in intestinal and hepatopancreas explant culture led to a concentration- and time-dependent modulation (mainly stimulatory) of most of the digestive enzymes tested. The ghrelin-induced upregulations of digestive enzyme expression were all abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6, and most of them by the phospholipase C inhibitor U73122 or the protein kinase A inhibitor H89. This indicates that ghrelin effects on digestive enzymes are mediated by GHS-R1a, partly by triggering the PLC/PKC and AC/PKA intracellular signaling pathways. These data suggest a role for ghrelin on digestive processes in fish.
Journal: Molecular and Cellular Endocrinology - Volume 442, 15 February 2017, Pages 165-181