کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5538907 | 1552358 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of the postruminal methionine requirement of growing lambs by using the indicator amino acid oxidation technique
ترجمه فارسی عنوان
بررسی نیاز متیونین پس از زایمان بره های رشد با استفاده از روش اکسیداسیون اسید آمینه
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Methionine was demonstrated to be the most limiting amino acid for growing sheep when microbial protein is the predominant source of absorbable AA, but its specific requirement was not well quantified. The indicator amino acid oxidation (IAAO) method is based on the concept that when the most limiting amino acid (LAA) is deficient for protein synthesis, then all other AAs will be oxidized. With increasing intakes of the LAA, IAAO will decrease until the requirement for the LAA is met, and there is no further change in the indicator oxidation afterwards. Therefore, the catabolism of 13C-Phe was used to indicate the effects of varying duodenal infusing levels of Met on the oxidation rate by 4-month-old Kazakh lambs (30.8 ± 0.4 kg BW). After a 6-d adaptation period, the IAAO test was conducted on d 7. Each lamb received a priming dose of 0.67 mg/kg BW of 13C-Phe injected via the jugular vein, together with 0.18 mg/kg BW of NaH13CO3 at time 0. Then, constant infusions [1.33 mg 13C-Phe/(kg h)â1] were started. Breath samples were collected prior to and half-hourly between 90 and 210 min after the start of the 13C-Phe infusions. An estimate of the mean Met requirement was determined by a breakpoint analysis of the rate of 13CO2 released by 13C-Phe oxidation, with a 2-phase linear regression model. The oxidation rate of 13C-Phe decreased (P < 0.001) with increasing amounts of methionine infused in a dose-dependent fashion up to 1.65 g/day, and it was not different from 1.65 to 2.15 g/day (P > 0.05). The mean Met requirement was estimated to be 1.28 ± 0.11 g/day of supplementation. The total postruminal minimum requirement of Met would be 2.02 g/day when the basal passage of Met (0.74 g/day) was summed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 228, June 2017, Pages 83-90
Journal: Animal Feed Science and Technology - Volume 228, June 2017, Pages 83-90
نویسندگان
Gao Wei, Liu Chen, Gao Xinmei, Zhang Fan, Chen Daofu, Liu Chenli,