کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540109 | 1553558 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Amphioxus miRNAs were identified via systematic analysis using deep RNA sequencing.
- Ten increased & 4 decreased immune-related miRNAs were identified using microarray.
- Three immune related amphioxus miRNAs were further validated by qRT-PCR.
- Immune-related amphioxus miRNAs were functional annotated.
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via regulating expressions of target genes at post-transcriptional level. Amphioxus, as modern survivor of an ancient chordate lineage, is a model organism for comparative genomics study. However, miRNAs involved in regulating immune responses in Branchiostoma belcheri are largely unclear. Here, we systematically investigated the microRNAs (miRNAs) involved in regulating immune responses in the cephalochordate amphioxus (Branchiostoma belcheri) through next-generation deep sequencing of amphioxus samples infected with Vibrio parahemolyticus. We identified 198 novel amphioxus miRNAs, consisting of 12 conserved miRNAs, 33 candidate star miRNAs and 153 potential amphioxus-specific-miRNAs. Using microarray profiling, 14 miRNAs were differentially expressed post infection, suggesting they are immune-related miRNAs. Eight miRNAs (bbe-miR-92a-3p, bbe-miR-92c-3p, bbe-miR-210-5p, bbe-miR-22-3p, bbe-miR-1â¼bbe-miR-133 and bbe-miR-217â¼bbe-miR-216 clusters) were significantly increased at 12Â h post-infection, while bbe-miR-2072-5p was downregulated at 6Â h and 12Â h. Three miRNAs, bbe-miR-1-3p, bbe-miR-22-3p and bbe-miR-92a-3p, were confirmed to be involved in immune responses to infection by qRT-PCR. Our findings further clarify important regulatory roles of miRNAs in the innate immune response to bacterial infection in amphioxus.
Journal: Developmental & Comparative Immunology - Volume 74, September 2017, Pages 243-252