کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5541712 1553846 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of commercial luminometry swabs for total bacteria and coliform counts in colostrum-feeding equipment
ترجمه فارسی عنوان
اعتبار سنجی سمبوم های لومومتری تجاری برای کل باکتری ها و شمار کولیروف در دستگاه های تغذیه شیردهی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
A sufficient quantity and quality of colostrum must be fed quickly to the newborn calf while minimizing bacterial contamination. Adenosine triphosphate bioluminescence swabs offer a potential rapid on-farm alternative to assess bacterial contamination of colostrum. The objective of this study was to validate the Hygiena (Camarillo, CA) AquaSnap Total (AS), SuperSnap (SS), PRO-Clean (PC), and MicroSnap Coliform (MS) swabs as well as visual hygiene assessment for detection of elevated bacterial counts in or on colostrum-feeding equipment. From April to October 2016, 18 esophageal tube feeders, 49 nipple bottles, and 6 pails from 52 dairy farms in Ontario were evaluated for cleanliness. Following visual hygiene assessment, sterile physiological saline (15 mL) was poured into each piece of equipment, mixed for 2 min to ensure total surface coverage, and poured into a sterile collection container through the feeding end. The fluid was split into equal aliquots, with one being evaluated by conventional culture and the other evaluated using the luminometry swabs. Nonparametric receiver operator curves were used to compare the test performance of the luminescence reading (relative light units; RLU) from each type of swab to conventional bacterial culture. The area under the curve comparing the AS swab to total bacterial count (cut point >100,000 cfu/mL) was 0.89, and using a cut point of 631 RLU correctly classified 84% of samples with a sensitivity of 88% and a specificity of 77%. The area under the curve comparing the MS swab to total coliform count (cut point >10,000 cfu/mL) was 0.85, and using a cut point of 44 RLU correctly classified 89% of samples with a sensitivity of 83% and a specificity of 90%. Visual hygiene assessment, PC and SS swabs were not reliable indicators for feeding equipment cleanliness. The results suggest that the AS and MS swabs can be used as an alternative to traditional laboratory bacterial counts to evaluate cleanliness of colostrum-feeding equipment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 100, Issue 11, November 2017, Pages 9459-9465
نویسندگان
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