کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5543904 | 1554297 | 2017 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of bioenergetic metabolism by the combination of metformin and 2-deoxyglucose highly decreases viability of feline mammary carcinoma cells
ترجمه فارسی عنوان
مهار متابولیسم انرژی زیستی با ترکیب متفورمین و 2-دگزیکس گوگلوزا به طور چشمگیری کاهش دهنده حیات سلول های کارسینوم ماموته زنانه است
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Feline mammary carcinoma (FMC) is a highly aggressive pathology that has been proposed as an interesting model of breast cancer disease, especially for the hormone refractory subgroup. Recently, cancer cell metabolism has been described as a hallmark of cancer cells. Here, we investigate the effects and mechanism of metabolic modulation by metformin (MET, anti-diabetic drug), 2-deoxyglucose (2DG, hexokinase inhibitor) or a combination of both drugs, MET/2DG on two established FMC cells lines: AlRB (HER2 (3Â +) and Ki67Â <Â 5%) and AlRATN (HER2 (â) and Ki67Â >Â 15%). We found that treatments significantly decreased both FMC cells viability by up to 80%. AlRB resulted more sensitive to 2DG than AlRATN (IC50: 3.15 vs 6.32Â mM, respectively). The combination of MET/2DG potentiated the effects of the individually added drugs on FMC cells. In addition, MET/2DG caused an increased in intracellular oxidants, autophagic vesicles and completely inhibited colony formation. Conversely, only MET significantly altered plasma membrane integrity, presented late apoptotic/necrotic cells and increased both glucose consumption and lactate concentration. Our results support further studies to investigate the potential use of this metabolic modulation approach in a clinical veterinary setting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 114, October 2017, Pages 461-468
Journal: Research in Veterinary Science - Volume 114, October 2017, Pages 461-468
نویسندگان
MarÃa Florencia Arbe, Chiara Fondello, Lucrecia Agnetti, Gabriel MartÃn Álvarez, MatÃas Nicolás Tellado, Gerardo Claudio Glikin, Liliana MarÃa Elena Finocchiaro, Marcela Solange Villaverde,