کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5545687 1555631 2017 46 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro treatment of Toxoplasma gondii with copper(II) complexes induces apoptosis-like and cellular division alterations
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
In vitro treatment of Toxoplasma gondii with copper(II) complexes induces apoptosis-like and cellular division alterations
چکیده انگلیسی
Toxoplasma gondii is the causative agent of toxoplasmosis, which is one of the most common parasitic diseases in the world. This pathogen causes severe damage to immunocompromised hosts, and the most frequently used therapy is the combination of pyrimethamine and sulfadiazine, which has side effects. Thus, there is a need for new therapies that target T. gondii. Herein, we present the anti-Toxoplasma effect of two new copper(II) complexes: [(H2L1) Cu (μ-Cl)2 Cu(H2L1)] Cl2·5H2O (1) and [(H2L2) Cu (μ-Cl)2 Cu(H2L2)] Cl2·6H2O (2). Complexes (1) and (2) irreversibly controlled parasite growth in vitro, with IC50 values of 0.78 μM and 3.57 μM, respectively, after 48 h. These complexes induced part of the tachyzoite population to convert to bradyzoites, which eventually die. The cell death mechanism was unknown, but signs of apoptosis, such as membrane blebs and nuclear fragmentation, and necrosis, such as plasma membrane disruption, intense cytoplasm vesiculation and the release of cellular contents, were seen. In addition, complex (2) interfered with the correct disposition of the inner membrane complex of the parasite, affecting cell division. These results indicate that these copper complexes have potential effects against T. gondii and may be used as drugs in the future or serve as prototypes for the development of new drugs to treat toxoplasmosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 245, 15 October 2017, Pages 141-152
نویسندگان
, , , , , , , ,