کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5545907 | 1555644 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA and protein profiles
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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![عکس صفحه اول مقاله: Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA and protein profiles Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA and protein profiles](/preview/png/5545907.png)
چکیده انگلیسی
The canine heartworm Dirofilaria immitis releases excretory/secretory molecules into its host and in culture. We report analyses of the types, amounts and stage-dependence of microRNAs and proteins found in D. immitis culture media recovered after incubating 800,000 microfilariae for 6Â days, 500Â L3 and 500Â L4 for 7Â days, as well as 40 adult females and 40 adult males for 48Â h, all separately. In addition, the presence of exosome-like particles was established by nanoparticle tracking analysis. Our results are in concordance with the D. immitis molecules previously detected in dog blood and in culture medium, but add additional insight into the sex- and stage-specificity of these processes. Of 131 miRNA candidates analyzed, none of the most abundant sequences was exclusively associated with one stage. Several isoforms of the nematode miR-100 family, miR-279, miR-71, were highly represented and overlapped substantially with the profile of heartworm miRNAs described from infected dog blood. lin-4 was primarily associated with males. We also report 4, 27 and 72 proteins in media from microfilariae, females and males, respectively. The only protein in common to all samples was actin, and only 9/88 proteins with a gene ontology description had not been reported in other studies of filarial secretomes. Exosomal proteins were well represented, dominated by cytoskeletal proteins, metabolic enzymes, zeta polypeptide, and chaperones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 232, 15 December 2016, Pages 1-7
Journal: Veterinary Parasitology - Volume 232, 15 December 2016, Pages 1-7
نویسندگان
Lucienne Tritten, Damian Clarke, Scott Timmins, Tom McTier, Timothy G. Geary,