کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5555306 1559740 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct effects of interleukin-8 on growth and functional activity of T lymphocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Direct effects of interleukin-8 on growth and functional activity of T lymphocytes
چکیده انگلیسی


- Activation of T-lymphocytes leads to an increase in T-cell production of IL-8.
- IL-8 is able to modulate IL-8 receptor expression on activated T cells.
- IL-8 is capable of affecting directly TCR-mediated activation of both CD4+ and СD4− T subsets.
- IL-8 is a modulator of cytokine production by activated T cells.

CD3+ T-lymphocytes were isolated from the normal donors by positive magnetic separation. Activation of the T cells with particles conjugated with antibodies to CD3, СD28 and СD2 molecules led to a marked increase in T-cell production of interleukine-8 (IL-8). We present evidence that IL-8 receptor α-chain (CXCR1, CD181) is expressed on the cell surface of 13.3% T cells. Activation of T-lymphocytes resulted in significant enhancement of CD181+ cells both in naive CD4+ T cell and terminally differentiated effector CD4+ T cell compartments with concomitant reduction of CD181+ cells in effector memory CD4+ T cell subset. The level of T cell activation was assessed judging from the surface expression of CD25 (IL-2 receptor α-chain). We demonstrate that IL-8 treatment (0.01-10.0 ng/ml concentration range) reduced the activation status of both CD4− and CD4+ effector memory T cells, as well as terminally differentiated effector T cells, without significantly affecting the activation of naive T cells or central memory T cells. In addition, IL-8 up-regulated IL-2 and down-regulated IL-10 production by activated T cells, with no effect on interferon-gamma (IFN-γ) and IL-4 production. Data obtained suggests the importance of IL-8 in the direct regulation of adaptive T cell reactivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 50, September 2017, Pages 178-185
نویسندگان
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