کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5556221 1560356 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr
چکیده انگلیسی

Ethnopharmacological relevanceEuphorbia pekinensis Rupr. (EP) is a Euphorbia species of Euphorbiaceae, which is widely used in traditional Chinese medicine. It has been reported to exhibit therapeutic effects on solid tumors, leukemias, and malignant ascites although underlying molecular mechanisms are poorly delineated. Anti-angiogenic therapy is a recognized strategy for treating cancer-based solid tumors, and is also associated with malignant ascites treatment.Study aimTo study the anti-angiogenic properties of the water extract of EP vinegar preparation (WEVEP).Materials and methodsFollowing WEVEP treatment, intersegmental blood vessels were assessed during the development of transgenic Tg (flk: mCherry) zebrafish as was the proliferation, migration and network formation of HUVECs in vitro. mRNA expression of specific angiogenic-related genes including VEGF family members, Met, and NRP2 was also measured using quantitative real-time PCR (Q-PCR).ResultsData demonstrated that angiogenesis was inhibited by the WEVEP in zebrafish (from 100 µg/mL to 250 µg/mL, p < 0.0001) and in the HUVEC model (from 100 µg/mL to 400 µg/mL, p < 0.0001). In the zebrafish model, the mean vessel numbers of administered groups were 26.00 ± 1.29 (100 µg/mL), 24.54 ± 2.20 (150 µg/mL), 22.66 ± 2.68 (200 µg/mL), 20.80 ± 1.75 (250 µg/mL), compared to 27.67 ± 0.96 of control group. Relative quantitative gene expression in zebrafish treated with WEVEP demonstrated that only VEGFR3 was significantly increased and other 23 genes including Met, VEGFA, Flt-1 were significantly decreased.ConclusionWEVEP can positively modulate angiogenesis via multiple targeting mechanisms. Our novel results contribute towards the discovery of a possible mechanism(s) of the traditional use of EP in the treatment of cancer and malignant ascites.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 206, 12 July 2017, Pages 337-346
نویسندگان
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