کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5558785 1403241 2016 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Successful Strategies to Determine High-Resolution Structures of GPCRs
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Successful Strategies to Determine High-Resolution Structures of GPCRs
چکیده انگلیسی
G protein-coupled receptors (GPCRs) constitute the largest class of drug targets in the human genome, which highlights the importance of understanding the molecular basis of their activation, downstream signaling, and regulation. Since 2007, great progress has been made in the field of GPCR structure determination and their signaling complexes at the molecular level. Here, we summarize the high-resolution structures of over 30 different GPCRs with their co-crystallized ligands, and outline the successful strategies involved, including construct design, expression systems, and lipidic cubic phase (LCP) composition, and the many key technical parameters of the crystallization methods. By comparing the success rates of different strategies used in the past, we wish to pave the road for more successful structure-function research for GPCRs in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Trends in Pharmacological Sciences - Volume 37, Issue 12, December 2016, Pages 1055-1069
نویسندگان
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