کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5559847 1561691 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immune response mechanism of mouse monocytes/macrophages treated with κ-carrageenan polysaccharide
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Immune response mechanism of mouse monocytes/macrophages treated with κ-carrageenan polysaccharide
چکیده انگلیسی


- Cell proliferation rate was highest treated with polysaccharide (100 μg/mL)at 24 h
- The mechanisms of immune function induced by polysaccharide were discussed.
- The immune response of RAW264.7 cells is via the TLR4-NF-κB pathway.

This study investigated the function of κ-carrageenan polysaccharide in immune regulation. The immune response of RAW 264.7 cells treated with κ-carrageenan polysaccharide was explored by MTT assay, general morphological observation, neutral red phagocytosis assay, Griess method, fluorescence method, and enzyme-linked immunosorbent assay. In addition, TLR4 blocking experiment and double-fluorescence immunostaining were performed on cells to demonstrate their immune response mechanism. Results show that κ-carrageenan polysaccharide not only promotes cell proliferation but also activates RAW 264.7 cells, thereby improving the cells' phagocytic capability, NO production, and tumor necrosis factor-α (TNF-α) secretion. In addition, the use of TLR4-specific inhibitors can significantly mediate the increased TNF-α secretion induced by κ-carrageenan polysaccharide. The RAW 264.7 cells treated with κ-carrageenan polysaccharide show upregulated TLR4 expression, and the main subunit of NF-κB (p65) is translocated. These results support the immunomodulatory function of κ-carrageenan polysaccharide in RAW 264.7 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 53, July 2017, Pages 191-198
نویسندگان
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