Novel feed including bioactive compounds from winery wastes improved broilers' redox status in blood and tissues of vital organs

- Grape pomace (GP), a polyphenolic winery by-product, was used for animals' feed preparation.
- The experimental feed was examined for its antioxidant activity in broilers.
- Broilers' feed supplemented with GP prevented free radical toxicity (e.g. lipid and protein oxidation).
- Feed supplemented with GP increased antioxidant capacity (e.g. increase in glutathione).

Currently, there is a great interest in the production of animal feed with antioxidant activity. The aim of this study was to examine the potential antioxidant effects of a feed supplemented with grape pomace (GP), a winery by-product with high environmental load, in chickens. Broilers of 15 days post birth were separated into two groups fed either with standard diet or with diet supplemented with GP for 35 days. Blood and tissues collections were performed after feeding for 15 and 35 days with the experimental diet (i.e. at 30 and 50 days post birth). Free radical toxicity markers, namely thiobarbituric acid reactive substances, protein carbonyls, total antioxidant capacity, reduced glutathione, catalase activity and rate of H2O2 decomposition were determined in blood and tissues of vital organs. The results indicated that feed supplemented with GP decreased oxidative stress-induced toxic effects and improved chickens' redox status, and so it may also improve their wellness and productivity. On the other hand, this exploitation of GP may solve problems of environmental pollution in areas with wineries.

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