کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5585446 | 1568203 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ISSR marker-assisted genetic diversity analysis of Dioscorea hispida and selection of the best variety for sustainable production
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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![عکس صفحه اول مقاله: ISSR marker-assisted genetic diversity analysis of Dioscorea hispida and selection of the best variety for sustainable production ISSR marker-assisted genetic diversity analysis of Dioscorea hispida and selection of the best variety for sustainable production](/preview/png/5585446.png)
چکیده انگلیسی
Plant breeding is a way of selection of a particular individual for the production of the progeny by separating or combining desired characteristics. The objective of this study was to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties using molecular techniques to select the best variety for sustainable production at the farmer's level. A total of 160 germplasms of Ubi gadong were collected from different locations at the Terengganu and Kelantan states of Malaysia. Forty eight (48) out of 160 germplasms were selected as “primary” selection based on yield and other qualitative characters. Selected collections were then grown and maintained for ISSR marker-assisted genetic diversity analysis. Overall plant growth and yield of tubers were also determined. A total of 12 ISSR markers were tested to justify the characteristics of Ubi gadong varieties among which three markers showed polymorphic bands and on average 57.3% polymorphism were observed representing the highest variation among germplasms. The ISSR marker based on UPGMA cluster analysis grouped all 48 D. hispida into 10 vital groups that proved a vast genetic variation among germplasm collections. Therefore, hybridization should be made between two distant populations. The D. hispida is already proved as the highest starch content tuber crops and very rich in vitamins with both micro and macro minerals. Considering all these criteria and results from marker-assisted diversity analysis, accessions that are far apart based on their genetic coefficient (like DH27 and DH71; DH30 and DH70; DH43 and DH62; DH45 and DH61; DH77 and DH61; DH78 and DH57) could be selected as parents for further breeding programs. This will bring about greater diversity, which will lead to high productive index in terms of increase in yield and overall quality and for the ultimate target of sustainable Ubi gadong production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comptes Rendus Biologies - Volume 340, Issue 8, August 2017, Pages 359-366
Journal: Comptes Rendus Biologies - Volume 340, Issue 8, August 2017, Pages 359-366
نویسندگان
Nur Fatihah Hasan Nudin, Abdul Manaf Ali, Norhayati Ngah, Nor Zuhailah Mazlan, Nashriyah Mat, Mohd Noor Abd. Ghani, Nadiawati Alias, Abd. Jamil Zakaria, Md. Sarwar Jahan,