کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5589167 1569808 2017 38 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MicroRNAs associated with drought response in the pulse crop common bean (Phaseolus vulgaris L.)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
MicroRNAs associated with drought response in the pulse crop common bean (Phaseolus vulgaris L.)
چکیده انگلیسی
Drought stress significantly reduces common bean yields. Recently, some drought-related miRNAs were found in various plants. However, reports of miRNAs involved in drought stress in common bean are limited. Here, we obtained four sRNA samples from drought-tolerant and -sensitive cultivars of common bean that experienced with or without drought treatment. A total of 49 novel miRNAs and 120 known miRNAs were detected. Under drought treatment, 9 and 7 known miRNAs were down and up-regulated, respectively, and 5 and 3 of the novel miRNAs were increased and decreased, respectively. Among these miRNAs, four miRNAs shared the same pattern of expression between Long 22-0579 and Naihua. Target genes of these miRNAs included transcription factors, protein kinases, and nuclear transcription factors. Finally, we verified all of the differentially expressed miRNAs by RT-qPCR, and we identified 16 miRNAs that are potentially associated with the drought stress response. These miRNAs and target genes will be useful in future basic studies and in applied studies investigating how miRNA regulation can be used to enhance drought resistance in plant species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 628, 10 September 2017, Pages 78-86
نویسندگان
, , ,