کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589539 | 1569805 | 2017 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis of flower development in tea (Camellia sinensis (L.))
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کلمات کلیدی
APETALA1ARFAP1FLCLFYSOC1RPKMFRICamellia sinensis (L.) O. KuntzeLATE ELONGATED HYPOCOTYLCoGVrn1Vrn2WRKYCCA1LHYELF4PIF3ELF3LuminidependensAP2ERFRNA-seqDEGsbHLHApetala 2 - آپتال 2basic helix-loop-helix - اسلحه پایه حلقه ایLEAFY - برگRNA sequencing - ترتیب RNAFlowering locus T - تلقیح توت TFlower development - توسعه گلconstants - ثابت هاgibberellic acid - جیبرلیک اسیدclusters of orthologous groups - خوشه های گروه های ارتوقیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوFMI - صندوق بین المللی پولTranscription factor - عامل رونویسیethylene response factor - عامل پاسخ اتیلنAuxin response factor - فاکتور پاسخ اکسینGene ontology - هستیشناسی ژنیDifferentially expressed genes - ژن های متفاوت بیان شده استcal - کالFLOWERING LOCUS C - گل LOCUS CCauliflower - گل کلمGIGANTEA - گیگنتا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, de novo transcriptome assembly and gene expression analysis using Illumina sequencing technology were performed. Transcriptomic analysis assembles gene-related information involved in reproductive growth of C. sinensis. Gene Ontology (GO) analysis of the annotated unigenes revealed that the majority of sequenced genes were associated with metabolic and cellular processes, cell and cell parts, catalytic activity and binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction were enriched among the DEGs. Furthermore, 207 flowering-associated unigenes were identified from our database. Some transcription factors, such as WRKY, ERF, bHLH, MYB and MADS-box were shown to be up-regulated in floral transition, which might play the role of progression of flowering. Furthermore, 14 genes were selected for confirmation of expression levels using quantitative real-time PCR (qRT-PCR). The comprehensive transcriptomic analysis presents fundamental information on the genes and pathways which are involved in flower development in C. sinensis. Our data also provided a useful database for further research of tea and other species of plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 631, 5 October 2017, Pages 39-51
Journal: Gene - Volume 631, 5 October 2017, Pages 39-51
نویسندگان
Feng Liu, Yu Wang, Zhaotang Ding, Lei Zhao, Jun Xiao, Linjun Wang, Shibo Ding,