کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589646 | 1569809 | 2017 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rice gene SDL/RNRS1, encoding the small subunit of ribonucleotide reductase, is required for chlorophyll synthesis and plant growth development
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کلمات کلیدی
SDLInDelEMSChlqRT-PCRAspartic acid - آسپارتیک اسدamino acid - آمینو اسیدethylmethane sulfonate - اتیل متان سولفوناتglutamic acid - اسید گلوتامیکTem - این استRice (Oryza sativa L.) - برنج (Oryza sativa L.)base pair - جفت پایهinsertion/deletion - درج / حذفChlorophyll - سبزینه یا کلروفیلChlorophyll synthesis - سنتز کلروفیلUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهTransmission electron microscopy - میکروسکوپ الکترونی عبوریGene mapping - نقشه برداری ژنhistidine - هیستیدینquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
A new mutant named sdl (stripe and drooping leaf) was characterized from indica cultivar Zhenong 34 by ethylmethane sulfonate (EMS) mutagenesis. The mutant sdl exhibited development defects including stripe and drooping leaf, dwarfism and deformed floral organs. The gene SDL was found allelic to RNRS1 by map-based cloning, which was homologous to Arabidopsis TSO2 encoding the small subunit of ribonucleotide reductase. The gDNA sequencing results of sdl in mutant showed that there was a repetitive sequence insertion of 138-bp at the 475th bp in the exon. The redundant sequence was conserved in SDL homologous proteins, which contained the active site (tyrosine), as well as two amino acids glutamate and histidine involved in the binding of iron. There were fewer chloroplasts and grana lamellas in sdl leaf compared with those of wild-type. Additionally, the stripe leaves of sdl seedlings were highly sensitive to temperature, since the chlorophyll content was increased with the temperature rising. The drooping leaf of sdl might be resulted from the disappearance of vascular bundles and mesophyll cells in both leaf midrib and lateral veins. Fittingly to the phenotypes of mutant sdl, the expression levels of genes associated with photosynthesis and chlorophyll synthesis were found to be down- or up-regulated at different temperatures in mutant sdl. Also, the transcriptional levels of genes related to plant height and floral organ formation showed obvious differences between wild-type and sdl. The “SDL/RNRS1” was, hence, required for the chlorophyll biosynthesis and also played pleiotropic roles in the regulation of plant development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 351-362
Journal: Gene - Volume 627, 5 September 2017, Pages 351-362
نویسندگان
Ran Qin, Dongdong Zeng, Rong Liang, Chengcong Yang, Delara Akhter, Md. Alamin, Xiaoli Jin, Chunhai Shi,