کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589704 | 1569824 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMAPPtRNAisopentenyl transferaseRNA polymerase II - آرانای پلیمراز II RNA polymerase III - RNA پلیمراز IIItRNA modification - اصلاح tRNAAmyloid fibers - الیاف آمیلوئیدtransfer ribonucleic acid - انتقال ریبونوکلئیک اسیدThT - بلهThioflavin T - تیوفلاوین Tdimethylallyl pyrophosphate - دی متیلللی پیرو فسفاتCongo red - سرخ کنگوPrion - پریونPol III - پل IIIPol II - پل دومRNA binding - پیوند RNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro](/preview/png/5589704.png)
چکیده انگلیسی
TRIT1 is a highly conserved tRNA isopentenyl transferase that modifies a subset of tRNAs in human cells and is a candidate tumor suppressor in lung cancer in certain ethnic populations. The yeast homologue, Mod5, has similar tRNA-modifying functions in the cytoplasm and is required for the transcriptional silencing activity of RNA polymerase II promoters near tRNA genes in the nucleus, a phenomenon termed tRNA gene mediated (tgm) silencing. Furthermore, Mod5 can fold into amyloid fibers in vitro and in vivo, which confers resistance to certain fungicides in yeast. Since TRIT1 complements both tRNA modifying and tgm-silencing activities in yeast where the Mod5 gene has been deleted, it seemed possible that TRIT1 might also have amyloid-forming capabilities. Here we show that TRIT1, like Mod5, directly binds to tRNAs that are both substrate and non-substrates for modification with similar affinity, and to an unstructured, non-tRNA. Binding appears to involve distinct protein-RNA multimers which decrease in electrophoretic mobility as the protein to RNA ratio increases. Furthermore, we characterize TRIT1 as a novel human amyloid fiber forming protein. We discuss these data in light of TRIT1's functional roles and possible implications for disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 612, 15 May 2017, Pages 19-24
Journal: Gene - Volume 612, 15 May 2017, Pages 19-24
نویسندگان
T.J. Waller, D.F. Read, D.R. Engelke, P.J. Smaldino,