کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590659 | 1570150 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of genome segments 2, 3 and 6 of epizootic hemorrhagic disease virus strains isolated in Japan in 1985-2013: Identification of their serotypes and geographical genetic types
ترجمه فارسی عنوان
شناسایی بخش های ژنوم 2، 3 و 6 سوسپانسیون ویروس بیماری های خونگرم اپیزیویی جدا شده در ژاپن در سال های 1985 تا 2013: شناسایی سروتایپ های آنها و انواع ژنتیک جغرافیایی
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کلمات کلیدی
CPESEGORFSNTSerum neutralization testEHDVEHDEpizootic hemorrhagic diseasedsRNAEpizootic hemorrhagic disease virusOIENTRdouble-stranded RNA - RNA دو رشته ایCytopathic effect - اثر سیتوپاتیکArbovirus - ارب ویروسSegment - بخشphylogenetic analysis - تجزیه و تحلیل فیلوژنتیکminimum essential medium - حداقل حداقل مورد نیازSerotype - سروتهopen reading frame - قاب خواندن بازMEM - مامانNon-translated region - منطقه غیر منتخبCattle - گاو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
چکیده انگلیسی
We characterized genome segments 2, 3 and 6 (Seg-2, Seg-3 and Seg-6) of 11 Japanese strains of epizootic hemorrhagic disease (EHD) virus (EHDV) isolated in 1985-2013. The Japanese strains were divisible into two groups based on phylogenetic analyses of the nucleotide sequences of Seg-2 and Seg-6. In both of the phylogenetic trees based on Seg-2 and Seg-6, seven of the 11 Japanese strains were grouped together with EHDV-2 and EHDV-7 strains, and the other four Japanese strains were grouped with EHDV-1 strains. The phylogenetic analysis of Seg-2 among EHDV strains identified 10 of the 11 Japanese strains as EHDV-1, EHDV-2 or EHDV-7. The other Japanese strain, ON-4/B/98, isolated from an asymptomatic cow in 1998 was in the same group as the EHDV-2 and EHDV-7 strains in the phylogenetic trees based on Seg-2 and Seg-6, but the results suggested that the strain belongs to another serotype. We thus conducted a serum neutralization test to identify that serotype by using anti-EHDV-2 and anti-EHDV-7 rabbit sera. We observed that the ON-4/B/98 strain was not sufficiently neutralized by any of the antisera, which suggests that the strain could be assigned into a new serotype, tentatively named 'EHDV-10.' Sequences of Seg-3 were also determined, and all of the Japanese strains were grouped together with Australian strains, suggesting that the Japanese strains are a part of EHDV distributed in the Asia-Pacific region. The data obtained herein would be beneficial for the diagnosis and prevention of EHD in Japan and neighboring countries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 53, September 2017, Pages 38-46
Journal: Infection, Genetics and Evolution - Volume 53, September 2017, Pages 38-46
نویسندگان
Hiroaki Shirafuji, Tomoko Kato, Makoto Yamakawa, Toru Tanaka, Yutaka Minemori, Tohru Yanase,