کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590703 | 1570157 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insights from molecular structure predictions of the infectious bronchitis virus S1 spike glycoprotein
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CTDRBDNTDInfectious bronchitis virus (IBV)IBVI-TASSERIterative Threading ASSEmbly RefinementC-terminal domain - دامنه C ترمینالN-terminal domain - دامنه N-terminalReceptor binding domain - دامنه گیرنده گیرندهInfectious bronchitis virus - ویروس برونشیت عفونیProtein structure prediction - پیش بینی ساختار پروتئینSpike glycoprotein - گلیکوپروتئین اسپایک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Infectious bronchitis virus is an important respiratory pathogen in chickens. The IBV S1 spike is a viral structural protein that is responsible for attachment to host receptors and is a major target for neutralizing antibodies. To date, there is no experimentally determined structure for the IBV S1 spike. In this study, we sought to find a predicted tertiary structure for IBV S1 using I-TASSER, which is an automated homology modeling platform. We found that the predicted structures obtained were robust and consistent with experimental data. For instance, we observed that all four residues (38, 43, 63, and 68) that have been shown to be critical for binding to host tissues, were found at the surface of the predicted structure of Massachusetts (Mass) S1 spike. Together with antigenicity index analysis, we were also able to show that Ma5 vaccine has higher antigenicity indices at residues close to the receptor-binding region than M41 vaccine, thereby providing a possible mechanism on how Ma5 achieves better protection against challenge. Examination of the predicted structure of the Arkansas IBV S1 spike also gave insights on the effect of polymorphisms at position 43 on the surface availability of receptor binding residues. This study showcases advancements in protein structure prediction and contributes useful, inexpensive tools to provide insights into the biology of IBV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 46, December 2016, Pages 124-129
Journal: Infection, Genetics and Evolution - Volume 46, December 2016, Pages 124-129
نویسندگان
Christina Lora M. Leyson, Brian J. Jordan, Mark W. Jackwood,