Expansion of IL-6+ Th17-like cells expressing TLRs correlates with microbial translocation and neurological disabilities in NMOSD patients

- High proportion of peripheral IL-17+ TLR+ CD4+ T cells was observed in NMOSD patients.
- The percentage of IL-17+ IL-6+ TLR+ CD4+ T cells was associated with severity of NMOSD.
- IL-17+ IL-6+ TLR+ CD4+ T cells were associated with plasma CD14 levels in NMOSD patients.
- Lower proportion of peripheral TLR+ Treg17 cells was observed in NMOSD patients.

Different microbial antigens, by signaling through toll-like receptors (TLR), may contribute to Th17-mediated autoimmune diseases, such as neuromyelitis optica spectrum disorder (NMOSD). The objective of this study was to determine the proportion of different Th17-like cell subsets that express TLR in NMOSD patients. For this study, the frequency of different Th17 cell subsets expressing TLR subsets in healthy individuals (n = 20) and NMOSD patients (n = 20) was evaluated by cytometry. The peripheral levels of soluble CD14 (sCD14) and cytokines were determined by ELISA. Our results demonstrated that the proportion of peripheral CD4+ T cells expressing TLR2, 4 and 9 was significantly higher in NMOSD samples than in healthy subjects. In NMOSD, these cells are CD28+ PD-1− CD57− and produce elevated levels of IL-17. Among different TLRs+ Th17-like subsets, the proportion of those that co-express IL-17 and IL-6 was significantly higher in NMOSD patients, which was positively correlated with sCD14 levels and EDSS score. By contrast, the percentage of TLRs+ Treg17 cells (IL-10+ IL-17+) was negatively related to sCD14 and the severity of NMOSD. In conclusion, the expansion of peripheral IL-6-producing TLR+ Th17-like cells in NMOSD patients was associated with both bacterial translocation and disease severity.

The percentage of IL-17+ IL-6+ TLR+ CD4+ T cell subset was high in NMOSD patients and its proportion was associated with severity of NMOSD.242