کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5666394 | 1407799 | 2016 | 7 صفحه PDF | دانلود رایگان |
To comprehensively examine the MICB gene polymorphism and identify its differences in Chinese Yao population from other ethnic groups, we investigated the polymorphism in the 5â²-upstream regulation region (5â²-URR), coding region (exons 2-4), and the 3â²-untranslated region (3â²-UTR) of MICB gene by using PCR-SBT method in 125 healthy unrelated Yao individuals in Guangxi Zhuang Autonomous Region. Higher polymorphism was observed in the 5â²-URR, nine single nucleotide polymorphisms (SNPs) and a two base pairs deletion at position â139/â138 were found in our study. Only five different variation sites, however, were detected in exons 2-4 and three were observed in the 3â²-UTR. The minor allele frequencies of all variants were greater than 5%, except for rs3828916, rs3131639, rs45627734, rs113620316, rs779737471, and the variation at position +11803 in the 3â²-UTR. The first nine SNPs of 5â²-URR and rs1065075, rs1051788 of the coding region showed significant linkage disequilibrium with each other. Ten different MICB extended haplotypes (EH) encompassing the 5â²-URR, exons 2-4, and 3â²-UTR were found in this population, and the most frequent was EH1 (23.2%). We provided several evidences for balancing selection effect on the 5â²-URR of MICB gene in Yao population.
Journal: Human Immunology - Volume 77, Issue 12, December 2016, Pages 1187-1193