کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5666937 1591738 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Blocking and dislocation of Candida albicans Cdr1p transporter by styrylquinolines
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Blocking and dislocation of Candida albicans Cdr1p transporter by styrylquinolines
چکیده انگلیسی


- Styrylquinolines show synergistic interaction with fluconazole and inhibit rhodamine 6G efflux from Candida albicans cells.
- The presence of styrylquinolines induces Cdr1p-GFP expression at protein level.
- Styrylquinolines act on the cell membrane of C. albicans; however, they do not affect Pma1p localization.

Styrylquinolines are a novel group of quinoline drugs that are known to have p53-independent antiproliferative activity and antiviral properties. This study evaluated the antifungal activity of these drugs more deeply, particularly their activity modulation towards Cdr1p, the main multidrug transporter of Candida albicans. Styrylquinolines were found to have antifungal activity and to work synergistically with fluconazole. Additionally, they decreased the extracellular concentration of rhodamine 6G in ABC-transporter-expressing cells. The cellular localization of GFP-tagged Cdr1p was assessed by epifluorescent microscopy. Styrylquinolines induce expression of Cdr1p, as confirmed by Western blotting. Three of four drugs tested caused the partial delocalization of transport protein to the cytoplasm. These results show the first evidence that styrylquinolines decrease the activity of ABC multidrug transporters in C. albicans.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Antimicrobial Agents - Volume 50, Issue 2, August 2017, Pages 171-176
نویسندگان
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