کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5667610 1407863 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of the calcium-sensing receptor in monocytes from synovial fluid is increased in osteoarthritis
ترجمه فارسی عنوان
بیان گرید گیرنده کلسیم در مونوسیت های مایع سینوویال در استئوآرتریت افزایش می یابد
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی ایمونولوژی، آلرژی و روماتولوژی
چکیده انگلیسی

ObjectivesWe assessed calcium-sensing receptor (CaSR) expression in monocytes isolated from synovial fluid of patients with different types of rheumatisms and explored whether CaSR expression was related to the inflammatory nature of synovial fluid.MethodsForty-one patients were included: osteoarthritis (n = 10), microcristallin rheumatisms (n = 10), rheumatoid arthritis (n = 12) and other inflammatory rheumatisms (n = 9). Surface and total CaSR expressions in monocytes isolated from synovial fluid and blood were assessed by flow cytometry analysis. U937 cells were cultured during 24 hours in presence of cell-free synovial fluids.ResultsEvery monocyte population tested express the CaSR intra- and extracellularly. Whereas similar pattern of CaSR expression exist in monocyte isolated from blood or synovial fluids, our results indicate that higher CaSR expression levels can be observed in monocytes from synovial fluids than in circulating monocytes. In both populations of monocytes, surface and total CaSR expressions were found to be significantly increased in patients with osteoarthritis compared to rheumatoid arthritis. Similar data were obtained when U937 cells were incubated with cell-free synovial fluids from osteoarthritis patients. Still present, this effect was significantly lowered when “inflammatory” synovial fluids were introduced in culture.ConclusionsOur results indicate that CaSR expression in synovial derived monocytes is higher in osteoarthritis than in inflammatory rheumatisms and that CaSR expression is modulated by the nature of the synovial fluid. Given the role played by monocytes in the pathogenesis of chronic rheumatisms, monocytes could be interesting therapeutic targets via the CaSR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Joint Bone Spine - Volume 84, Issue 2, March 2017, Pages 175-181
نویسندگان
, , , , , , , , ,