کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5667990 1592337 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Automated processing, extraction and detection of herpes simplex virus types 1 and 2: A comparative evaluation of three commercial platforms using clinical specimens
ترجمه فارسی عنوان
پردازش خودکار، استخراج و تشخیص ویروس هرپس سیمپلکس انواع 1 و 2: ارزیابی مقایسه سه سیستم تجاری با استفاده از نمونه های بالینی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- This is the first study to compare the performance of three, commercially-available automated systems for the detection and differentiation of HSV types 1 and 2 from clinical specimens.
- This study utilized a consensus reference standard, defined as the result achieved by at least 3 of the 4 molecular methods. This is advantageous over past studies that have used viral culture as a reference standard, which typically underestimates the specificity of molecular methods.
- The three commercial assays and automated platforms provide high overall agreement (>97%; kappa >0.9) for the qualitative detection and differentiation of HSV-1/2.

BackgroundRecently, automated platforms have been developed that can perform processing, extraction and testing for herpes simplex virus (HSV) nucleic acid on a single instrument.ObjectivesIn this study, we compared three commercially-available systems; Aptima®/Panther (Hologic, San Diego, CA), ARIES® (Luminex Corporation, Austin, TX), and cobas® 4800 (Roche Molecular Systems Inc, Pleasanton, CA) for the qualitative detection of HSV-1/2 in clinical samples.Study designTwo-hundred seventy-seven specimens (genital [n = 193], dermal [n = 84]) were submitted for routine HSV-1/2 real-time PCR by a laboratory developed test. Following routine testing, samples were also tested by the Aptima, ARIES, and cobas HSV-1/2 assays per the manufacturer's recommendations. Results were compared to a “consensus standard” defined as the result obtained from ≥3 of the 4 assays.ResultsFollowing testing of 277 specimens, the cobas and ARIES assays demonstrated a sensitivity of 100% for HSV-1 (61/61) and HSV-2 (55/55). The Aptima assays showed a sensitivity of 91.8% (56/61) for HSV-1 and 90.9% (50/55) for HSV-2. Percent specificities for HSV-1 were 96.2% (202/210) by cobas, 99.5% (209/210) by ARIES and 100% (236/236) by Aptima. For HSV-2, the specificities were 98.1% (211/215) by cobas, 99.5% (215/216) by ARIES and 100% (216/216) by Aptima. The turnaround time for testing 24 samples was 2.5 h by the cobas 4800, 3.1 h by Aptima/Panther, and 3.9 h by ARIES.ConclusionsThe three commercial systems can perform all current functions on a single platform, thereby improving workflow and potentially reducing errors associated with manual processing of samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 89, April 2017, Pages 30-33
نویسندگان
, , , , ,