کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5668089 1592333 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High prevalence of cytomegalovirus infection in surgical intestinal specimens from infants with necrotizing enterocolitis and spontaneous intestinal perforation: A retrospective observational study
ترجمه فارسی عنوان
شیوع بالای عفونت سیتومگالوویروس در نمونه های روده جراحی از نوزادان مبتلا به انتروکولیت نکروتیک و پرفوراسیون روده خود به خود: مطالعه ی مشاهدات گذشته نگر
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- To our knowledge, this is the first study to evaluate the prevalence of CMV infection in infants with NEC, SIP, and associated surgical conditions in a larger cohort of patients.
- We found CMV proteins and nucleic acids in intestinal specimens from a high percentage of infants with these diseases.
- Our findings shed light on mechanisms by which CMV might contribute to the pathogenesis and progression of these diseases or exacerbate their severity.

BackgroundNecrotizing enterocolitis (NEC) is a severe, often fatal gastrointestinal emergency that predominantly affects preterm infants, and there is evidence that neonatal cytomegalovirus (CMV) infection may in some cases contribute to its pathogenesis.ObjectivesThis study aimed to evaluate the prevalence of CMV in infants with NEC.Study designSeventy intestinal specimens from 61 infants with NEC, spontaneous intestinal perforation (SIP), or related surgical complications were collected at Karolinska University Hospital and Uppsala University Hospital, Sweden. Ten specimens from autopsied infants without bowel disease served as controls. Samples were analyzed for CMV immediate-early antigen (IEA), CMV late antigen (LA), 5-lipoxigenase (5LO) and CMV-DNA by immunohistochemistry (IHC) and in situ hybridization (ISH), respectively. In 10 index samples, CMV DNA was analyzed with Taqman PCR after laser capture microdissection (LCM) of cells positive for CMV IEA by IHC.ResultsCMV IEA was detected by IHC in 57 (81%) and CMV LA in 45 (64%) of 70 intestinal specimens from index cases; 2 (20%) of 10 control specimens were positive for both antigens. 5LO was detected in intestinal tissue section obtained from all examined index and controls. CMV DNA was detected in 4 of 10 samples (40%) after LCM. By ISH, all 13 IHC-IEA-positive samples were positive for CMV DNA; however, 3 of 5 IHC-IEA-negative samples (60%) were also positive.ConclusionsCMV-specific antigens and CMV DNA were highly prevalent in intestinal specimens from infants with NEC, SIP, and related surgical complications. Our findings provide further evidence that neonatal CMV infection contributes to the pathogenesis of these diseases and may affect patient outcome.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 93, August 2017, Pages 57-64
نویسندگان
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