کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5668874 | 1407924 | 2017 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Azithromycin Modulates 3â²,5â²-cyclic Diguanylic Acid Signaling in Pseudomonas aeruginosa Azithromycin Modulates 3â²,5â²-cyclic Diguanylic Acid Signaling in Pseudomonas aeruginosa](/preview/png/5668874.png)
Macrolides have been reported to exert a variety of effects on both host immunomodulation and repression of bacterial pathogenicity. In this study, we report that the 3â²,5â²-cyclic diguanylic acid (c-di-GMP) signaling system, which regulates virulence in Pseudomonas aeruginosa, is affected by the macrolide azithromycin. Using DNA microarray analysis, we selected a gene encoding PA2567 related to c-di-GMP metabolism that was significantly affected by azithromycin treatment. Expression of the PA2567 gene was significantly repressed by azithromycin in a time- and dose-dependent manner, whereas no difference in PA2567 gene expression was observed in the absence of azithromycin. In-frame deletion of the PA2567 gene affected both virulence factors and the quorum-sensing system, and significantly decreased total bacteria in a mouse pneumonia model compared to the wild-type strain (P < 0.05). These results suggest that macrolides possess the ability to modulate c-di-GMP intracellular signaling in P. aeruginosa.
Journal: Journal of Infection and Chemotherapy - Volume 23, Issue 8, August 2017, Pages 550-555