کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
571874 | 1452605 | 2015 | 10 صفحه PDF | دانلود رایگان |
• Comparison of CEN.PK and S288c for production of vanillin glucoside
• S288c produces 2-fold more vanillin glucoside than CEN.PK in batch fermentation
• S288c produces 10-fold more vanillin glucoside than CEN.PK in continuous cultivation
• The higher yield by S288c is achieved during respiratory growth
• Strain background evaluation should be performed early in a metabolic strategy
The yeast Saccharomyces cerevisiae is a widely used eukaryotic model organism and a key cell factory for production of biofuels and wide range of chemicals. From the broad palette of available yeast strains, the most popular are those derived from laboratory strain S288c and the industrially relevant CEN.PK strain series. Importantly, in recent years these two strains have been subjected to comparative “-omics” analyzes pointing out significant genotypic and phenotypic differences. It is therefore possible that the two strains differ significantly with respect to their potential as cell factories for production of specific compounds. To examine this possibility, we have reconstructed a de novo vanillin-β-glucoside pathway in an identical manner in S288c and CEN.PK strains. Characterization of the two resulting strains in two standard conditions revealed that the S288c background strain produced up to 10-fold higher amounts of vanillin-β-glucoside compared to CEN.PK. This study demonstrates that yeast strain background may play a major role in the outcome of newly developed cell factories for production of a given product.
Journal: Metabolic Engineering Communications - Volume 2, December 2015, Pages 99–108