کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5736588 | 1613768 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of neural stem cell media on hypoxic injury in rat hippocampal slice cultures
ترجمه فارسی عنوان
اثرات محیطی سلولهای بنیادی عصبی بر آسیبهای هیپوکسیک در کشت تیرهای هیپوکامپ موش صحرایی
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کلمات کلیدی
NT4FGF1IGF2NT3HIFNGFGCSFGDNFGLUT1Igf1 - IGF1Neural stem cells - سلولهای بنیادی عصبیFibroblast growth factor 1 - عامل رشد فیبروبلاست 1granulocyte colony-stimulating factor - فاکتور تحریک کننده کلنی گرانولوسیتVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)nerve growth factor - فاکتور رشد عصبinsulin-like growth factor 1 - فاکتور رشد مانند انسولین 1insulin-like growth factor 2 - فاکتور رشد مانند انسولین 2Hypoxia Inducible Factor - فاکتور قابل تشخیص هیپوکسیglial cell-derived neurotrophic factor - فاکتور نوروپاتیک مشتق شده از سلول گلیالBrain - مغزNeurotrophin 3 - نوروتروفین 3Neurotrophin 4 - نوروتروفین 4Hippocampus - هیپوکامپ Hypoxia - هیپوکسیglucose transporter 1 - گلوکز 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Neonatal hypoxic-ischemic brain injuries cause serious neurological sequelae, yet there is currently no effective treatment for them. We hypothesized that neurotrophic factors released into the medium by stem cells could supply hypoxia-damaged organotypic hippocampal slice cultures with regenerative abilities. We prepared organotypic slice cultures of the hippocampus of 7-day-old Sprague-Dawley rats based on the modified Stoppini method; slices were cultured for 14Â days in vitro using either Gahwiler's medium (G-medium) or stem cell-conditioned medium (S-medium) as culture medium. At day 14 in vitro, hippocampal slice cultures were exposed to 95% N2 and 5% CO2 for 3Â h to induce hypoxic damage, the extent of which was then measured using propidium iodide fluorescence and immunohistochemistry images. We performed dot blotting to estimate neurotrophic/growth factor levels in the G- and S-media. Organotypic hippocampal slices cultured using S-medium after hypoxic injury were significantly less damaged than those cultured using G-medium. GLUT1, NGF, GDNF, VEGF, GCSF, and IGF2 levels were higher in S-medium than in G-medium, whereas FGF1, HIF, and MCP3 levels were not significantly different between media. In conclusion, we found that stem cell-conditioned medium had a neuroprotective effect against hypoxic injury, and that, of the various neurotrophic factors in S-medium, NGF, GDNF, and VEGF can contribute to neuroprotection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1677, 15 December 2017, Pages 20-25
Journal: Brain Research - Volume 1677, 15 December 2017, Pages 20-25
نویسندگان
Na Mi Lee, Soo Ahn Chae, Hong Jun Lee,