کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737608 | 1614721 | 2017 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of dopamine D1 receptors enhances the temporal summation and excitability of rat retinal ganglion cells
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کلمات کلیدی
OPLINLKirIPLPKCmEPSCsRGCsPLCInward rectifying K+ channelGCLTTXaCSFEGTAHEPES4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidEPSP - epspiadenylate cyclase - آدنیلات سیکلاسethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیس (β-آمینویل اتر) -N، N، N '، N'-tetraacetic اسیدtetrodotoxin - تترو دوتوکسین excitability - تحریک پذیریminiature excitatory postsynaptic currents - جریانهای پسینپتیک هیجان انگیز مینیاتوریTemporal summation - جمع بندی موقتیDopamine - دوپامینRetinal ganglion cells - سلول های گانگلیونی شبکیهretinal ganglion cell - سلول گانگلیونی شبکیهphospholipase C - فسفولیپاز Couter plexiform layer - لایه بیرونی بیرونیinner plexiform layer - لایه داخلی لایهinner nuclear layer - لایه داخلی هسته ایganglion cell layer - لایه سلول گانگلیونیartificial cerebral spinal fluid - مایع مغزی نخاعی مغزیexcitatory postsynaptic potential - پتانسیل پست سیناپتی هیجان انگیزProtein kinase C - پروتئین کیناز سیD1 receptors - گیرنده های D1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activation of dopamine D1 receptors enhances the temporal summation and excitability of rat retinal ganglion cells Activation of dopamine D1 receptors enhances the temporal summation and excitability of rat retinal ganglion cells](/preview/png/5737608.png)
چکیده انگلیسی
Dopamine (DA), an important neurotransmitter and neuromodulator, plays important roles in neuronal physiological functions by activating G-protein-coupled DA D1 and/or D2 receptors. Previous studies have demonstrated that D1 receptors are functionally expressed in retinal neurons and glial cells, including ganglion cells. In this study, we explored the effects of D1 receptor activation on retinal ganglion cell (RGC) temporal summation and excitability in rat retinal slices using electrophysiological techniques. Bath application of the selective D1 receptor agonist SKF81297 increased the ratio of excitatory postsynaptic potentials (EPSPs) (EPSP5/EPSP1) within an EPSP train evoked by a train stimulation (five current pulses at 40Â Hz), which was blocked by co-application of SCH23390, a specific D1 receptor antagonist. Ba2+, an inwardly rectifying K+ channel (Kir) blocker, significantly suppressed the SKF81297-induced effect, whereas ZD7288, a specific hyperpolarization-activated cation current (Ih) blocker, showed a moderate inhibitory effect. The cAMP/protein kinase A (PKA) signaling pathway, but not phosphoinositide-specific phospholipase C (PI-PLC), mediated the SKF81297-induced modulation of EPSP temporal summation. Further experiments showed that SKF81297 suppressed Ba2+-sensitive Kir currents in RGCs. Additionally, SKF81297 increased the spontaneous firing frequency of RGCs, and caused depolarization of the cells with or without the presence of synaptic receptor blockers. In contrast, SKF81297 did not significantly change the frequency of miniature excitatory postsynaptic currents (mEPSCs) recorded in RGCs. Our results indicate that D1 receptor activation enhances the temporal summation of RGCs mainly by suppressing Kir currents through the cAMP/PKA signaling pathway, thus increasing the excitability of rat RGCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 355, 4 July 2017, Pages 71-83
Journal: Neuroscience - Volume 355, 4 July 2017, Pages 71-83
نویسندگان
Peng Cui, Xue-Yan Li, Yuan Zhao, Qian Li, Feng Gao, Ling-Zhu Li, Ning Yin, Xing-Huai Sun, Zhongfeng Wang,