کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737706 | 1614727 | 2017 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Serotonin regulation in a rat model of exercise-induced chronic fatigue
ترجمه فارسی عنوان
تنظیم سرتوونین در یک مدل رت از خستگی مزمن ناشی از ورزش
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کلمات کلیدی
5-HTHPLC-MS5-hydroxytryptamineBCAAs5-HTT5-HIAA5-HT1A5-Hydroxyindoleacetic acid - 5-هیدروکسی سدیم اسیدbranched-chain amino acids - آمینو اسیدهای زنجیرهای منشعب شدهTryptophan - تریپتوفان5-HT transporter - حمل کننده 5-HTCNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیhigh-performance liquid chromatography-mass spectrometry - طیف سنجی جرم کروماتوگرافی مایع با کارایی بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
This study investigated the mechanisms underlying regulation of the serotonin system in the rat brain during exercise-induced chronic fatigue. High-performance liquid chromatography-mass spectrometry (HPLC-MS) was performed to measure serum tryptophan of the fatigued rat. HPLC was conducted to measure 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in the frontal cortex and hippocampus. In addition, 5-HT1A receptor and 5-HT transporter (5-HTT) mRNA expressions were measured at the same locations using real-time PCR. The results demonstrated a significant reduction in the serum tryptophan level in rats with exercise-induced chronic fatigue. Moreover, increased 5-HT and decreased 5-HIAA levels were detected in the frontal cortex and hippocampus, and these alterations were significant. Further, 5-HTT expression was significantly increased and 5-HT1A receptor expression was significantly decreased. These results indicate that the 5-HT system plays an important role in the development of exercise-induced chronic fatigue. The 5-HT levels in different parts of the brain increased simultaneously, especially at synapses, and these alterations were associated with changes in 5-HTT and 5-HT1A mRNA expressions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 349, 4 May 2017, Pages 27-34
Journal: Neuroscience - Volume 349, 4 May 2017, Pages 27-34
نویسندگان
Zhandong Liu, Yanjue Wu, Tianhui Liu, Ren Li, Minhao Xie,