کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737790 | 1614728 | 2017 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis reveals differential activation of microglial genes after ischemic stroke in mice
ترجمه فارسی عنوان
تجزیه و تحلیل ترانسکتومیک نشان دهنده فعال سازی دیفرانسیل ژن های میکروگلالی پس از سکته مغزی ایسکمیک در موش است
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کلمات کلیدی
PBSCSF1RCSF1qRTMGINLRsPFAMCP-1DGEColony stimulating factor 1 receptorMouse Genome InformaticsTLRs - TLR هاCellular components - اجزای سلولیdigital gene expression - بیان ژن دیجیتالMolecular function - تابع مولکولیTranscriptome - ترانسکریپتومKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوStroke - سکته مغزیcolony stimulating factor - عامل تحریک کننده کلنیPhotothrombosis - عوارض جانبیBiological processes - فرآیندهای بیولوژیکیActivation - فعال سازیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroglia - میکروگلیاهاGene ontology - هستیشناسی ژنیparaformaldehyde - پارافرمالدهیدMonocyte chemotactic protein-1 - پروتئین chemotactic monocyte-1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Microglia are immune cells in the brain and play a pivotal role in the progression of ischemic injury, but the gene expression and signaling pathways related to the activation of microglia following ischemia remain unclear. In our experiment, we used digital gene expression (DGE) analysis to profile the transcriptome of ischemic tissue in a photothrombosis model. DGE analysis identified that a total of 749 genes were differentially regulated (643 up-regulated and 106 down-regulated) after 2Â days and 7Â days following stroke. We found 74.5% of these differentially expressed genes were microglial genes. Gene ontology (GO) analysis categorizes these differentially expressed genes at 2Â days and 7Â days to specific biological processes such as inflammatory response, cell activation, cell proliferation, and chemokine and cytokine production. Our data demonstrated that a large number of microglial genes were highly regulated at 2Â days after stroke, but the number of differentially expressed genes had reduced drastically by 7Â days. Importantly, some of the differentially expressed microglial genes at 7Â days did not show differential expression at 2Â days after stroke. DGE analysis indicated that specific genes related to microgliosis were regulated after ischemia. Consistent with the changes in transcriptome, the results from histological analysis of transgenic mice revealed that the microglia proliferated and aggregated surrounding the ischemic core during the period from 2Â days to 7Â days following photothrombosis. Together, these results suggested that transcriptomic changes in microglial genes after stroke may have a profound implication for pathophysiology and treatment of stroke.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 348, 21 April 2017, Pages 212-227
Journal: Neuroscience - Volume 348, 21 April 2017, Pages 212-227
نویسندگان
Akbar Khan, Furong Ju, Wenguang Xie, Muhammad Tariq Hafeez, Xiaofeng Cheng, Zhijie Yang, Lirui Zhu, Ting Li, Shengxiang Zhang,