کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737791 | 1614728 | 2017 | 47 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resveratrol protects against spinal cord injury by activating autophagy and inhibiting apoptosis mediated by the SIRT1/AMPK signaling pathway
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کلمات کلیدی
sirtuin 1TBSTPFART-PCRAMPKDMEMLPSPBSSirt1DMSO - DMSODulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoTris-buffered saline with Tween 20 - Tris-buffered saline با Tween 20Spinal cord injury - آسیب نخاعیAutophagy - اتوفاژیSprague–Dawley - اسپراگ داولیBasso, Beattie, and Bresnahan - باسو، بیتی و برسنهانBax - باکسRoom temperature - دمای اتاقDimethyl sulfoxide - دیمتیل سولفواکسیدResveratrol - رسوراترولBBB - سد خونی مغزیLipopolysaccharides - لیپوپلی ساکارید هاPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHematoxylin and Eosin - هماتوکسیلین و ائوزینreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسparaformaldehyde - پارافرمالدهیدBcl-2 Associated X protein - پروتئین Bcl-2 Associated X
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Resveratrol protects against spinal cord injury by activating autophagy and inhibiting apoptosis mediated by the SIRT1/AMPK signaling pathway Resveratrol protects against spinal cord injury by activating autophagy and inhibiting apoptosis mediated by the SIRT1/AMPK signaling pathway](/preview/png/5737791.png)
چکیده انگلیسی
Spinal cord injury (SCI) is a devastating condition with few effective treatments. Resveratrol, a polyphenolic compound, has exhibited neuroprotective effects in many neurodegenerative diseases. However, the explicit effect and mechanism of resveratrol on SCI is still unclear. Adenosine 5â² monophosphate-activated protein kinase (AMPK) and Sirtuin 1 (SIRT1), the downstream protein, play key roles in metabolizing of energy, resisting of resistance, and cellular protein homeostasis. In this study, we determined the effects of resveratrol on SCI and their potential relationship with SIRT1/AMPK signaling pathway, autophagy and apoptosis. To determine the effect of resveratrol on SCI recovery, a spinal cord contusion model was employed. Rats received treatment with resveratrol or DMSO immediately following contusion. We determined that Basso, Beattie, and Bresnahan (BBB) scores were significantly higher for injured rats treated with resveratrol. Nissl and HE staining revealed that resveratrol treatment significantly reduced the loss of motor neurons and lesion size in the spinal cord of injured rats when compared to vehicle-treated animals. Spinal cord tissue was assessed by Western blot, reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analyses 7Â days after injury for changes in expression of SIRT1/AMPK signaling pathway, autophagy and apoptosis proteins. Expression of SIRT1, p-AMPK, Beclin-1, LC3-B, and Bcl-2 was elevated in resveratrol-treated animals, whereas expression of p62, Cleaved Caspase-3, Caspase-9, and Bcl-2 associated X protein (Bax) was inhibited. Immunofluorescence analysis of primary neurons treated with resveratrol alone or in combination with Compound C (AMPK inhibitor) or EX527 (SIRT1 inhibitor) revealed that treatment with the inhibitors blocks the increased LC3-B expression in cells and increases the portion of TUNEL-positive cells. Taken together, these results suggest that resveratrol exerts neuroprotective effects on SCI by regulating autophagy and apoptosis mediated by the SIRT1-AMPK signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 348, 21 April 2017, Pages 241-251
Journal: Neuroscience - Volume 348, 21 April 2017, Pages 241-251
نویسندگان
Haosen Zhao, Shurui Chen, Kai Gao, Zipeng Zhou, Chen Wang, Zhaoliang Shen, Yue Guo, Zhuo Li, Zhanghui Wan, Chang Liu, Xifan Mei,