A novel intrinsic electroretinogram response in isolated mouse retina

Since the discovery of intrinsic photosensitive retinal ganglion cell (ipRGC) was reported in 2002, many features specific to this cell type have been described. However, scare information is available on the retinographic components directly reflecting ipRGC activity. In this study, we identified the electroretinogram (microERG) that reflects the photoresponses by ipRGCs in ex vivo preparations of the mouse retina, in which classical photoreceptors (cones and rods) were ablated mechanically and photochemically. MicroERG consisted of three components: a large transient ON response, a small and lazy hump 19 s after the onset of the light, and a large transient OFF response. A complete microERG recording required at least 30 s of light exposure. MicroERG showed the highest spectral photosensitivity at 478 nm. This wavelength corresponds to the peak wavelength in the ipRGCs' photosensitive curve. The psychophysical test using a blue light-emitting diode (LED) light (470 nm) revealed that the absolute threshold illuminance for microERG was greater than 12.26 log photons/s/cm2 in both ON and OFF responses, whereas microERG was not adapted for dark. The amplitude of microERG increased linearly with irradiance. The sensitivity of temporal frequency was high in microERG (at least 100 Hz), as suggested by the study on melatonin suppression by flickering light in human subjects (Zelter et al., 2014). Melatonin secretion was suppressed by light via ipRGCs and the suprachiasmatic nucleus. These properties of the photoresponse indicate that microERG may reflect the functions of ipRGC as a luminance detector in the mouse retina.