کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737903 | 1614725 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exposure to diphtheria toxin during the juvenile period impairs both inner and outer hair cells in C57BL/6 mice
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کلمات کلیدی
EF-2PBSHB-EGFABRHEPESDTRSGNSPLOHCPFA4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidH&E - H & EEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدHearing loss - از دست دادن شنواییDegeneration - انحطاطIHC - ایمونوهیستوشیمیphosphate buffer - بافر فسفاتdiphtheria toxin - توکسین دیفتریCochlea - حلزون گوشSound pressure level - سطح فشار صوتouter hair cell - سلول بیرونی موInner hair cell - سلول داخلی داخلیhair cell - سلول موOtotoxicity - سمیت زایی برای گوش، اتوتوکسیسیتیelongation factor-2 - ضریب انقباض 2Stria vascularis - عروق کرونرheparin-binding epidermal growth factor-like growth factor - فاکتور رشد مانند فاکتور رشد اپیدرمی هپارین استPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیScanning electron microscopy - میکروسکوپ الکترونی روبشیspiral ganglion neuron - نورون گانگلیون مارپیچیwildtype - نوع وحشیHematoxylin and Eosin - هماتوکسیلین و ائوزینparaformaldehyde - پارافرمالدهیدauditory brainstem response - پاسخ شنوایی مغزPostnatal - پس از زایمان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Exposure to diphtheria toxin during the juvenile period impairs both inner and outer hair cells in C57BL/6 mice Exposure to diphtheria toxin during the juvenile period impairs both inner and outer hair cells in C57BL/6 mice](/preview/png/5737903.png)
چکیده انگلیسی
Diphtheria toxin (DT) administration into transgenic mice that express the DT receptor (DTR) under control of specific promoters is often used for cell ablation studies in vivo. Because DTR is not expressed in mice, DT injection has been assumed to be nontoxic to cells in vivo. In this study, we demonstrated that DT application during the juvenile stage leads to hearing loss in wild-type mice. Auditory brainstem response measurement showed severe hearing loss in C57BL/6 mice administered DT during the juvenile period, and the hearing loss persisted into adulthood. However, ototoxicity did not occur when DT was applied on postnatal day 28 or later. Histological studies demonstrated that hearing loss was accompanied by significant degeneration of inner and outer hair cells (HCs), as well as spiral ganglion neurons. Scanning electron microscopy showed quick degeneration of inner HCs within 3Â days and gradual degeneration of outer HCs within 1Â week. These results demonstrated that DT has ototoxic action on C57BL/6 mice during the juvenile period, but not thereafter, and the hearing loss was due to degeneration of inner and outer HCs by unknown DT-related mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 351, 20 May 2017, Pages 15-23
Journal: Neuroscience - Volume 351, 20 May 2017, Pages 15-23
نویسندگان
Hiroyuki Konishi, Nobutaka Ohgami, Aika Matsushita, Yuki Kondo, Yuki Aoyama, Masaaki Kobayashi, Taku Nagai, Shinya Ugawa, Kiyofumi Yamada, Masashi Kato, Hiroshi Kiyama,