کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5738616 1615045 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperProfiling of cytosolic and mitochondrial H2O2 production using the H2O2-sensitive protein HyPer in LPS-induced microglia cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research paperProfiling of cytosolic and mitochondrial H2O2 production using the H2O2-sensitive protein HyPer in LPS-induced microglia cells
چکیده انگلیسی


- Subcellular H2O2 formation was confirmed using the HyPer in BV-2 microglia.
- Cytosolic H2O2 started increasing constantly from early time in response to LPS.
- Mitochondrial H2O2 rapidly increased at later time by LPS treatment.
- MAPK, such as JNK and p38 affected cytosolic H2O2, but not mitochondrial H2O2.

Dysregulation of the production of pro-inflammatory mediators in microglia exacerbates the pathologic process of neurodegenerative disease. ROS actively affect microglia activation by regulating transcription factors that control the expression of pro-inflammatory genes. However, accurate information regarding the function of ROS in different subcellular organelles has not yet been established. Here, we analyzed the pattern of cytosolic and mitochondrial H2O2 formation in LPS-activated BV-2 microglia using the H2O2-sensitive protein HyPer targeted to specific subcellular compartments. Our results show that from an early time, cytosolic H2O2 started increasing constantly, whereas mitochondrial H2O2 rapidly increased later. In addition, we found that MAPK affected cytosolic H2O2, but not mitochondrial H2O2. Consequently, our study provides the basic information about subcellular H2O2 generation in activated microglia, and a useful tool for investigating molecular targets that can modulate neuroinflammatory responses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 654, 27 July 2017, Pages 6-11
نویسندگان
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