کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5738822 1615059 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chloride conducting light activated channel GtACR2 can produce both cessation of firing and generation of action potentials in cortical neurons in response to light
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Chloride conducting light activated channel GtACR2 can produce both cessation of firing and generation of action potentials in cortical neurons in response to light
چکیده انگلیسی


- Light activated chloride channel GtACR2 was expressed in vivo in rat pyramidal neurons.
- Light stimulation of GtACR2 can induce both inhibition and excitation of cells.
- Light-induced generation of action potentials most likely occurs in axonal terminals.
- GtACR2 potentially could be used both as cell silencer and cell activator in optogenetics experiments.

Optogenetics is a powerful technique in neuroscience that provided a great success in studying the brain functions during the last decade. Progress of optogenetics crucially depends on development of new molecular tools. Light-activated cation-conducting channelrhodopsin2 was widely used for excitation of cells since the emergence of optogenetics. In 2015 a family of natural light activated chloride channels GtACR was identified which appeared to be a very promising tool for using in optogenetics experiments as a cell silencer. Here we examined properties of GtACR2 channel expressed in the rat layer 2/3 pyramidal neurons by means of in utero electroporation. We have found that despite strong inhibition the light stimulation of GtACR2-positive neurons can surprisingly lead to generation of action potentials, presumably initiated in the axonal terminals. Thus, when using the GtACR2 in optogenetics experiments, its ability to induce action potentials should be taken into account. Our results also open an interesting possibility of using the GtACR2 both as cell silencer and cell activator in the same experiment varying the pattern of light stimulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 640, 15 February 2017, Pages 76-80
نویسندگان
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