Impact of potent bioremediation enhancing plant extracts on Raoultella ornithinolytica properties

- Changes of fatty acid responsible for stabilizing membrane structure.
- Saponins cause changes in the structure of bacterial cultures.
- High concentrations of saponins on bacteria can be inducer of point mutations of genes.
- Contact bacteria with saponins led to cell surface modification.

Long-term contact of microorganisms with different compounds in the environment can cause significant changes in cell metabolism. Surfactants adsorption on cell surface or incorporation in the cell membrane, lead to their modification, which helps microorganisms adopt to the conditions of metabolic stress. The main objective of this study was to investigate the effects of three saponin-reach plant extracts from Hedera helix, Saponaria officinalis and Sapindus mucorossi on growth and adaptation of Raoultella ornithinolytica to high concentrations of these substances. For this purpose we investigated cell surface properties, membrane fatty acids and genetic changes of the microorganisms. The results revealed that prolonged exposure of the microorganisms to high concentrations of these surfactants can induce genetic changes of their genes. Moreover, the adaptation to contact with high concentrations of saponins was also associated with changes in composition of fatty acids responsible for the stabilisation of membrane structure and the increase in membrane permeability. The changes affected also the outer layer of cells. A significant increase (p < 0.05) in the cell surface hydrophobicity of tested strain was also observed. The cells after long-term contact with S. officinalis and S. mucorossi acquire properties that may be favourable in hydrophobic substances bioremediation.