کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5748861 1619145 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus
چکیده انگلیسی


- The antioxidant system was firstly activated and then inhibited by Cr(VI).
- Cr(VI) induced ROS production and promoted lipid peroxidation.
- Cr(VI) induced apoptosis via mitochondrial dysfunction and metacaspase activation.
- Cr(VI) induced early apoptosis followed by late apoptosis.
- Cr(VI) induced K+ effluxes and overload of cytoplasmic and mitochondrial Ca2+.

White rot fungi have been proved to be a promising option for the removal of heavy metals, understanding their toxic response to heavy metals is conducive to developing and popularizing fungi-based remediation technologies so as to lessen the hazard of heavy metals. In this study, Cr(VI)-induced oxidative stress and apoptosis in Pycnoporus sanguineus, a species of white rot fungi were investigated. The results suggested that high level of Cr(VI) promoted the formation of ROS, including H2O2, O2
- − and ·OH. With the increment of Cr(VI) concentration, the SOD and CAT activity along with GSH content increased within the first 24 h, but decreased afterward, companied with a significant enhancement of MDA content. Cr(VI)-induced oxidative damage further caused and aggravated apoptosis in P. sanguineus, especially at Cr(VI) concentrations above 20 mg/L. Cr(VI)-induced apoptosis was involved with mitochondrial dysfunction including mitochondrial depolarization, the enhancement of mitochondrial permeability and release of cytochrome c. The early and late apoptosis hallmarks, such as metacaspase activation, phosphatidylserine (PS) externalization, DNA fragmentation and the nuclear condensation and fragmentation were observed. Moreover, we also found disturbances of ion homeostasis, which was featured by K+ effluxes and overload of cytoplasmic and mitochondrial Ca2+.Based on these results, we suggest that Cr(VI) induced oxidative stress and apoptosis in white rot fungi, P. sanguineus.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 228, September 2017, Pages 128-139
نویسندگان
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