کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5749247 1412476 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of perfluorooctanoic acid on proteomic expression and cell membrane fatty acid of Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Influence of perfluorooctanoic acid on proteomic expression and cell membrane fatty acid of Escherichia coli
چکیده انگلیسی


- Relation between fatty acids and proteomics in E. coli exposed to PFOA was revealed.
- Palmitoleic acid was the biomarker reflected PFOA stress.
- Up-regulation of major catalytic enzymes involved in carbohydrate, lipid metabolism.
- Down-regulated proteins inhabited transport and energy metabolism under PFOA stress.
- Promotion of TCA cycle and fatty acid biosynthesis increased membrane permeability.

Perfluorooctanoic acid (PFOA) has received an increasing attention in the agricultural and food industries due to its risk to human health. To facilitate the development of novel biomarkers of Escherichia coli against PFOA through multi-omics technologies, and to reveal the resistance mechanism of E. coli against PFOA at protein levels, the interactions among pollutant stress, protein expression and cell metabolism was investigated by using iTRAQ-based quantitative proteomic analysis. The results revealed that the 63 up-regulated proteins mainly involved in tricarboxylic acid cycle, glyoxylate and dicarboxylate metabolism and fatty acid biosynthesis, whereas, the 69 down-regulated proteins related to oxidative phosphorylation, pyruvate metabolism and the cell cycle-caulobacter pathway, were also associated with the increase of membrane permeability, excessive expenditure of ATP, disruption of fatty acid biosynthesis under PFOA stress. The results provide novel insights into the influence mechanisms of PFOA on fatty acid and protein networks.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 220, Part A, January 2017, Pages 532-539
نویسندگان
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