کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
576124 | 1453074 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative cytotoxicity and accumulation of Roxarsone and its photodegradates in freshwater Protozoan Tetrahymena thermophila
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کلمات کلیدی
Inorganic arsenite(IC50)(PBS)(SDS-PAGE)sodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدRoxarsone - رکسارسونHydride generation-atomic fluorescence spectrometry - طیف سنجی فلورسانس اتمی نسل هیدریدattenuated total reflectance Fourier transform infrared spectroscopy - طیف سنجی مادون قرمز تبدیل فوریه تضعیف بازتاب کاملmedian inhibitory concentration - غلظت بازدارنده متوسطPhosphate buffered saline - فسفات بافر شورmedian effective concentration - متوسط غلظت موثر
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بهداشت و امنیت شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparative cytotoxicity and accumulation of Roxarsone and its photodegradates in freshwater Protozoan Tetrahymena thermophila Comparative cytotoxicity and accumulation of Roxarsone and its photodegradates in freshwater Protozoan Tetrahymena thermophila](/preview/png/576124.png)
چکیده انگلیسی
Roxarsone (ROX) remains to be as an organoarsenical feed additive used widely in developing countries. However, most of the ROX is excreted unchanged in manure, which could be readily photodegraded into inorganic arsenic derivatives. In this study, the comparative cytotoxicity and arsenic accumulation were evaluated after the exposure of Tetrahymena thermophila (T. thermophila) cell model to ROX and its photodegradates. The cytotoxic effects were estimated according to the relevant cell growth curves, morphologies and MTT assays. The 36Â h median effective concentrations for ROX and its photodegradates at various photolysis times (10, 20, and 30Â min) are 39.0, 2.08, 1.88, and 1.82Â mg (total arsenic) Lâ1, respectively. In parallel, the cellular arsenic uptakes were determined by hydride generation-atomic fluorescence spectrometry. Phospholipid layer as basic membrane structure was mimicked to assess the correlation between membrane permeability and cytotoxicity. The biocompatibility of ROX was dependent on its tendency to interact with cell membrane while the cytotoxicity was induced by the trans-membrane of the inorganic arsenic species present in the photodegradates of ROX. Furthermore, the photodegradates of ROX-associated alterations of intracellular protein profiles were analyzed using a proteomic approach. Overall, the significance was clarified that the control of arsenic emission caused by the application of ROX needs to be imposed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 286, 9 April 2015, Pages 171-178
Journal: Journal of Hazardous Materials - Volume 286, 9 April 2015, Pages 171-178
نویسندگان
Wenzhong Zhang, Fang Xu, Jingjing Han, Qun Sun, Kai Yang,