کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5761832 | 1624802 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structural characterization of a novel oligosaccharide from Achyranthes bidentata and its anti-osteoporosis activities
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Achyranthes bidentata Blume is one of the most frequently used medicinal herbs prescribed for treatment of osteoporosis in China. This study describes the crude saccharide (AB90) from A. bidentata and reports the osteoprotective effects of AB90 in ovariectomized (OVX) rats. The results indicated that AB90 significantly increased the bone mineral content (BMC) and bone mineral density (BMD) and improved the biomechanical properties in OVX rats. To further explore the fraction(s) responsible for above activity, a novel oligosaccharide (ABW90-1) was isolated continuously by DEAE-Cellulose 52 and Sephacryl S-100 filtration columns and characterized by infrared spectroscopy, monosaccharide analysis, methylation and NMR analysis. It was composed of (2 â 6)-linked-β-d-fructofuranosyl (Fruf), (2 â 1)-linked-β-d-Fruf residues and was terminated with Glc and Fru residues. The advanced structure and conformation of novel polysaccharide ABW90-1 were investigated by circular dichroism (CD) and scanning electron microscopy (SEM) data, and the results indicated that ABW90-1 was composed of randomly distributed sheet-like appearances instead of a triple-helix structure. In addition, in vitro activity of ABW90-1 was evaluated by using primary osteoblasts, and the data suggested that ABW90-1 exhibited favorable effects on the proliferation and differentiation of primary osteoblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 108, 1 December 2017, Pages 458-469
Journal: Industrial Crops and Products - Volume 108, 1 December 2017, Pages 458-469
نویسندگان
Changsheng Wang, Dawei Zhang, Mengliu Zhang, Yukun Jiao, Keming Jiang, Chunyan Yan,