کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5762048 1624803 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding
چکیده انگلیسی
Flow cytometry is a useful tool for determining ploidy levels of plants. One objective of this study was to estimate the rate of apomixis occurring in different USDA germplasm lines, as well as whether or not there was a meiotic reduction in the megaspore mother cell, and if there was fertilization to form an embryo. We examined the leaf tissue of maternal plants and 100 progeny seeds from these plants, to estimate their rate of apomixis. Combined ploidy information from two different seed tissues (embryo and endosperm) was utilized. Flow cytometric analysis of polyhaploids was also conducted to enable validation of their mode of reproduction, which have not previously been identified in the literature. Results from this study indicated that off-type progeny (seed) would not have been identified if only leaf samples were used, resulting in either, an over-estimation of the rate of apomixis for the accession, or misclassification of the type of reproduction. All identified polyhaploids were analyzed for genetic diversity, and were compared with existing diploid germplasm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 107, 15 November 2017, Pages 618-623
نویسندگان
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