کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5762172 | 1624810 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Somatic embryogenesis from sepal explants in Sapindus trifoliatus, a plant valuable in herbal soap industry
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کلمات کلیدی
IAANaAPFDBAPPGRs2, 4-D6-Benzylaminopurine - 6-بنزیلامینوپورینMurashige and Skoog (1962) - Murashige و Skoog (1962)indole-3-acetic acid - اسید Indole-3-استیکphoton flux density - تراکم شار فوتونPlant growth regulators - تنظیم کننده های رشد گیاهیSomatic embryo - جنین سمیMicropropagation - ریز ازدیادیnaphthalene acetic acid - نفتالین استیک اسیدKin - کینkinetin - کینتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Somatic embryogenesis and complete plant regeneration were achieved in callus cultures derived from sepal explants of Sapindus trifoliatus, an important and valuable tree used in saponin and herbal soap industry. Embryogenic calli were induced on MS medium containing 5.0 mg lâ1 2, 4-D and 0.1 mg lâ1 Kin. Explant orientation markedly influenced on somatic embryogenesis. The calli induced on medium supplemented with 2, 4-D and Kin showed the formation of whitish or greenish nodular embryogenic structure on MS basal medium containing l-glutamine. These nodular embryogenic structures gave rise to somatic embryos or secondary nodular embryogenic structures or both. Nodular embryogenic structures separated from embryogenic calli and transferred to MS medium containing 200 mg lâ1l-glutamine produced maximum number of globular, heart and cotyledonary stage somatic embryos. Histological studies have revealed the development of different stages of somatic embryos from nodular embryogenic structure. The embryogenic competence of the culture could be maintained by recurrent production of nodular embryogenic structures and somatic embryos. Somatic embryos were germinated and converted into plantlet on MS basal medium containing 2% sucrose. Plants propagated in vitro from somatic embryos were hardened and successfully established in field condition. The improved and promising regeneration method reported here for the S. trifoliatus may be valuable in herbal soap industry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 100, June 2017, Pages 228-235
Journal: Industrial Crops and Products - Volume 100, June 2017, Pages 228-235
نویسندگان
Pooja Asthana, Manoj K. Rai, Uma Jaiswal,