کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5764108 | 1625914 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Estrogen alters gonadal soma-derived factor (Gsdf)/Foxl2 expression levels in the testes associated with testis-ova differentiation in adult medaka, Oryzias latipes
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کلمات کلیدی
qPCRGonadal soma-derived factorGsdfDMYDMRT1foxl2EDCPBSQuantitative PCR - PCR کمیestradiol benzoate - استرادیول بنزواتEstrogen - استروژنAMH - اوSertoli cell - سلول سرتولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMedaka - مداکاEndocrine Disrupting Chemicals - مواد شیمیایی خرابکار غدد درون ریزAnti-Müllerian hormone - هورمون Anti-Mellerian
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Testis-ova differentiation in sexually mature male medaka (Oryzias latipes) is easily induced by estrogenic chemicals, indicating that spermatogonia persist in sexual bipotentiality, even in mature testes in medaka. By contrast, the effects of estrogen on testicular somatic cells associated with testis-ova differentiation in medaka remain unclear. In this study, we focused on the dynamics of sex-related genes (Gsdf, Dmrt1, and Foxl2) expressed in Sertoli cells in the mature testes of adult medaka during estrogen-induced testis-ova differentiation. When mature male medaka were exposed to estradiol benzoate (EB; 800Â ng/L), testis-ova first appeared after EB treatment for 14Â days (observed as the first oocytes of the leptotene-zygotene stage). However, the testis remained structurally unchanged, even after EB treatment for 28Â days. Although Foxl2 is a female-specific sex gene, EB treatment for 7Â days induced Foxl2/FOXL2 expression in all Sertoli cell-enclosed spermatogonia before testis-ova first appeared; however, Foxl2 was not detected in somatic cells in control testes. Conversely, Sertoli-cell-specific Gsdf mRNA expression levels significantly decreased after EB treatment for 14Â days, and no changes were observed in DMRT1 localization following EB treatment, whereas Dmrt1 mRNA levels increased significantly. Furthermore, after EB exposure, FOXl2 and DMRT1 were co-localized in Sertoli cells during testis-ova differentiation, although FOXL2 localization was undetectable in Sertoli-cell-enclosed apoptotic testis-ova, whereas DMRT1 remained localized in Sertoli cells. These results indicated for the first time that based on the expression of female-specific sex genes, feminization of Sertoli cells precedes testis-ova differentiation induced by estrogen in mature testes in medaka; however, complete feminization of Sertoli cells was not induced in this study. Additionally, it is suggested strongly that Foxl2 and Gsdf expression constitute potential molecular markers for evaluating the effects of estrogenic chemicals on testicular somatic cells associated with estrogen-induced testis-ova differentiation in mature male medaka.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 191, October 2017, Pages 209-218
Journal: Aquatic Toxicology - Volume 191, October 2017, Pages 209-218
نویسندگان
Tohru Kobayashi, Ayaka Chiba, Tadashi Sato, Taijun Myosho, Jun Yamamoto, Tetsuro Okamura, Yuta Onishi, Mitsuru Sakaizumi, Satoshi Hamaguchi, Taisen Iguchi, Yoshifumi Horie,