Enrichment of denitrifying methanotrophic bacteria from municipal wastewater sludge in a membrane bioreactor at 20 °C

- Denitrifying methanotrophic bacteria were enriched from municipal wastewater sludge.
- The maximum denitrification rate at 20 °C was similar to maximum rates at 30 °C.
- After 1-2 years consumption rates decreased; the cause for this is unknown.
- Results indicate higher rates may be achieved by addition of low amounts of copper.
- Increased rates may also be achieved at shorter hydraulic retention times.