کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5767964 1413211 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Encapsulation of lutein in liposomes using supercritical carbon dioxide
ترجمه فارسی عنوان
کپسول سازی لوتئین در لیپوزوم ها با استفاده از دی اکسید کربن فوق بحرانی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Lutein-loaded liposomes were prepared using supercritical carbon dioxide (SC-CO2).
- Liposomes have a small particle size and high encapsulation efficiency (EE).
- High pressure and depressurization rate increase the EE and uniformity of liposomes.
- Amphiphiles may be uniformly dispersed in an aqueous phase upon depressurization.
- The SC-CO2 method showed great promise to produce liposomes to deliver bioactives.

Liposomes loaded with lutein were prepared utilizing supercritical carbon dioxide (SC-CO2). The effects of pressure, depressurization rate, temperature and lutein-to-lipid ratio on particle size distribution, zeta potential, encapsulation efficiency (EE), bioactive loading, morphology, phase transition and crystallinity were investigated. Liposomes prepared by the SC-CO2 method had a particle size of 147.6 ± 1.9 nm-195.4 ± 2.3 nm, an encapsulation efficiency of 56.7 ± 0.7%-97.0 ± 0.8% and a zeta potential of −54.5 ± 1.2 mV to −61.7 ± 0.6 mV. A higher pressure (200-300 bar) and depressurization rate (90-200 bar/min) promoted a higher encapsulation of lutein whereas the lutein-to-lipid ratio had the dominant effect on the morphology of vesicles along with size distribution and EE. X-ray diffraction data implied a substantial drop in the crystallinity of lutein upon its redistribution in the liposome membranes. Differential scanning calorimetry indicated a broadened phase transition upon the simultaneous rearrangement of lutein and phospholipid molecules into liposomal vesicles. The SC-CO2 method resulted in particle characteristics highly associated with the ability of CO2 to disperse phospholipids and lutein molecules. It offers a promising approach to use dense phase CO2 to homogenize hydrophobic or amphiphilic aggregates suspended in an aqueous medium and regulate the vesicular characteristics via pressure and depressurization rate. The SC-CO2 method has potential for scalable production of liposomal nanovesicles with desirable characteristics and free of organic solvents.

202

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 100, Part 1, October 2017, Pages 168-179
نویسندگان
, , , ,