Molecular dissection of mercury-responsive transcriptome and sense/antisense genes in Medicago truncatula

► Genome-wide sequencing of Hg-exposed M. truncatula was conducted to analyze gene expression and complexity. ► Millions of sequenced tags were obtained from Hg-exposed libraries. ► Most of the annotated genes were expressed differentially under Hg stress. ► GO and KEGG analysis showed modified molecular functions and metabolic pathways.