Effect of fly ash on biochemical responses and DNA damage in earthworm, Dichogaster curgensis

► Biochemical estimation suggests fly ash induces ROS production in the earthworms. ► Fly ash affects lysosomal membrane stability in terms of NRR time. ► Comet assay demonstrates DNA damage (OTM) in coelomocytes due to fly ash exposure. ► Formation of DNA-protein crosslinks was observed in earthworm coelomocytes.